HYDROCARBONOCLASTIC POTENTIAL OF BACTERIA ISOLATED FROM HAIR SALON WASTEWATER IMPACTED SOILS
DOI:
https://doi.org/10.60787/Keywords:
Hydrocarbonoclastic bacteria, dichlorophenol indophenol indicator, salon wastewater, polluted soil,Abstract
The hydrocarbonoclastic potential of bacteria isolated from hair salon wastewater-impacted soils was studied. Isolation of hydrocarbonoclastic bacteria from hair salon wastewater-impacted soil (SWS) and non-salon wastewater-impacted soil (NWS) samples were determined. Total mean bacterial counts of the soil samples were carried out using standard microbiological techniques. The total bacterial counts were 1.2x106 ±0.5 and 8.0 ±0.2 x105 Cfu g-1 in the SWS and NWS, respectively. Hydrocarbonoclastic bacteria identified included Staphylococcus, Bacillus, Pseudomonas, Micrococcus, and Streptococcus spp. Utilization of some hydrocarbons tests were carried out on kerosene, petrol, diesel, and waste engine oil on the isolates under aerobic conditions and were determined using Dichlorophenol Indophenol indicator (DCPIP) in hydrocarbon medium and incubated for 48 hours at 30 C. Biosurfactants production potential using the drop collapse test indicated that most of the bacterial isolates which tested positive have good bio-surfactants production potentials. The result of the emulsification index, E24 % of diesel and petrol for Staphylococcus sp. was significantly higher (50.70± 1.21 and 51.50± 0.50 respectively) than that obtained for kerosene and waster-engine oil (31.40± 1.05 and 23.47± 0.57 respectively) (p<0.05).
In contrast, the amount of waste engine oil was significantly less than that of kerosene. There was no significant difference in emulsification index (p<0.05) between diesel (50.70± 1.21) and petrol (51.50± 0.50). Salon wastewater-impacted soils have been shown in this study to harbour bacteria with hydrocarbonoclastic potentials as indicated by the emulsification index E24% of the different isolates. The results suggest that the isolates are potential candidates for hydrocarbon utilization studies and application in bioremediation.
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