Study of hydrocarbonoclastic bacteria isolated from salon wastewater impacted soils
DOI:
https://doi.org/10.60787/Keywords:
Hydrocarbonoclastic bacteria, dichlorophenol indophenol indicator, salon wastewater, polluted soil,Abstract
ABSTRACT Isolation of hydrocarbonoclastic bacteria from hair salon effluent impacted soil (SEs) and non-salon effluent impacted soil (NSs) samples were determined. Total mean bacterial counts of the soil samples carried out using standard microbiological pour plate method on nutrient agar were 1.2x106 ±0.5 and 8.0 ±0.2 x105Cfu g-1 in the SEs and NSs respectively while identified hydrocarbonoclastic bacteria include species of Staphylococcus, Bacillus, Pseudomonas, Micrococcus and Streptococcus .Utilization of some hydrocarbon:kerosene, petrol, diesel and waste engine oil by the isolates under aerobic conditions was determined using Dichlorophenol Indophenol indicator (DCPIP) in hydrocarbon medium and incubated for 48hours at 30oC. Biosurfactants production potential using the drop collapse test indicated that most of the bacterial isolates which tested positive have good biosurfactants production potentials. Result of the emulsification index ,E24 % of diesel and petrol for Staphylococcus sp. was significantly higher than that obtained for kerosene and waster-engine oil (p<0.05) while that of waste engine oil was significantly less than that of the kerosene but between diesel and petrol, there was no significant difference in emulsification index(p<0.05). Salon wastewater impacted soils have been shown in this study to harbour bacteria with hydrocarbonoclastic potentials as indicated by the emulsification index E24% of the different isolates. The results suggest that the isolates are potential candidates for hydrocarbon utilization studies and application in bioremediation. Keywords: Dichlorophenol Indophenol indicator, hydrocarbonoclastic bacteria, polluted soil, hydrocarbon products, salon effluent.
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