COMPARATIVE ANALYSIS OF STRIGOLACTONE PRODUCTION IN BAMBARA GROUNDNUT AND COWPEA GENOTYPES
Akwapoly Journal of Communication and Scientific Research (APJOCASR)
DOI:
https://doi.org/10.60787/apjocasr.Vol8no1.24Keywords:
Bambara groundnut, Alectra vogelii, Striga generioides, strigolactones, orobanchol, fabacyl acetate., orobanchyl acetateAbstract
This study examined strigolactones (SLs) in 12 Bambara groundnut root exudates. A mixture of three strigolactones, orobanchol, orobanchyl acetate, and fabacyl acetate, were detected by LC-MS/MS in the root exudates of all Bambara groundnut genotypes investigated. Fabacyl acetate was not detected over ten days of P-starvation. Two of these strigolactones, orobanchol and orobanchyl acetate, were previously identified in cowpeas. The levels of orobanchol and orobanchyl acetate secreted varied significantly between genotypes (p<0.001) and (p<0.04), respectively. Over 21 days of P-starvation, meagre amounts of fabacyl acetate (< 10-12 M) were detected in Bambara groundnut root exudates, and there were no significant differences between genotypes. Among all the genotypes studied, Mana was the highest producer of the strigolactones detected, while DodR was the genotype whose exudates contained the lowest amount of strigolactones. The relative proportion of orobanchol contained in the strigolactone mixture of root exudates was very high across all 12 genotypes. These detected SL mixtures induced the germination of A. vogelii seeds at concentrations as low as 10-12 M but did not lead to S. gesnerioides germination, suggesting that A. vogelii is more sensitive to SL induction than S.gesnerioides and therefore differences inSL quantity maybe a key factor determining host specificity of parasitic plants. Although Alectrabutnot Striga parasitized the 12 BGN genotypes studied in a separate TETFund IBR grant awarded to the Federal Polytechnic Ekowe, genotypes with lower concentrations of SL mixtures result in lower Alectra shoot counts, suggesting that Bambara groundnut may be resistance/tolerance to root parasitic plants based on reduced SL production.
References
Akiyama, K., Ogasawara, S, Ito, S., Hayashi, H. (2005). Structural requirements of strigolactones for hyphal branching in AM fungi. Plant & Cell Physiology 51, 1104-1117. Bambara Groundnut Production in Singida and Dodoma Regions, Tanzania. Advances in Research,1-8.
Bouwmeester, H. J. (2011). Quantification of the relationship b/w strigolactones and Striga hermonthica infection in rice under varying levels of nitrogen and phosphorus. Weed Research, 51(4), 373-385.
Bouwmeester, H. J., Roux, C., Lopez-Raez, J. A., & Becard, G. (2007). Rhizosphere comm of plants, parasitic plants and AM fungi. Trends in plant science, 12(5), 224- 230.
Bouwmeester, H.J., Matusova, R., Zhongkui, S., Beale, M. H., (2003). Secondary metabolite signalling in host-parasitic plant interactions. Current Opinion in Plant Biology 6, 358-364.
Ebert, A. W. (2014). Potential of Underutilized Traditional Vegetables and Legume Crops to Contribute to Food and Nutritional Security, Ent- 2′-epi-orobanchol and its acetate, as germination stimulants for Striga gesnerioides Seeds isolated from cowpea and red clover. Journal of agricultural and food chemistry, 59 (19), 10485-10490.
Fernández-Aparicio, M., Kisugi, T., Xie, X., Rubiales, D., & Yoneyama, K. (2014). Low strigolactone root exudation: a novel mechanism of broomrape (Orobanche and Phelipanche spp.) resistance available for fababean breeding. Journal of Agricultural and Food chemistry, 62(29), 7063-7071.
Genetic variation in strigolactone production and tillering in rice and its effect on Striga hermonthica infection. Planta, 235(3), 473-484.
Gurney, A. L., Slate, J., Press, M. C., & Scholes, J. D. (2006). A novel form of resistance in rice to the angiosperm parasite Striga hermonthica. New phytologist, 169(1), 199-208.
H. (2007). Phosphorus deficiency in red clover promotes exudation of orobanchol, the signal for Income and More Sustainable Production Systems. Sustainability 6 (1): 319-
Jamil, M., VanMourik, T. A., Charnikhova, T., & Bouwmeester, H. J. (2013). Effect of diammonium phosphate application on strigolactone production and Strigahermonthica infection in three sorghum cultivars. Weed Research, 53(2), 121-130.
K.(2008). Isolation and identification of alectrolas (+)- obanchylacetate, a Germination stimulant for root parasitic plants. Phytochem, 69 (2), 427-431.
Kamara, A. Y., Chikoye, D., Ekeleme, F., Omoigui, L. O., & Dugje, I. Y. (2008). Field performance of improved cowpea varieties under conditions of natural infestation by the parasitic weed Strigagesnerioides. International Journal of Pest Management, 54(3),189-195.
Kenya, 24-30 June 1991.(pp. 303-305). CIMMYT (International Maize and Wheat Improvement Center).
Jarvis, A., Rieseberg, L. H. & Struik, P. C. (2014). Increasing homogeneity in global food supplies and the implications for food security. Proceedings of the National Academy of Sciences of the United States of America 111(11): 4001- 4006.
Kohlen, W., De Vos, R.,...& Bouwmeester, H. (2008). Tomato strigolactones are derived from carotenoids and their biosynthesis is promoted by phosphate starvation. New Phytologist, 178(4), 863-874.
Mbega, E. R., Massawe, C. R., & Mbwaga, A. M. (2016). Alectra vogelii, a Threat Bouwmeester, H. J. (2018). Genetic variation in Sorghum bicolor strigolactones and their role in resistance against Striga hermonthica. Journal of experimental botany, 69(9), 2415-2430.
Müller, S., Hauck, C., & Schildknecht, H. (1992). Germination stimulants produced by Vigna unguiculata Walp cv Saunders Upright. Journal of Plant Growth Regulation, 11(2), 77.
Muranaka,S., Mizutani, M., Takikawa, H., & Sugimoto, Y. (2011). Mycorrhizal symbionts and germination stimulant for root parasites. Planta, 225(4), 1031-1038
Omoigui, L. O., Kamara, A. Y., Ishiyaku, M. F., & Boukar, O. (2010). Comparative responses of cowpea breeding lines to Striga and Alectrainthedry Savanna of northeast Nigeria. African Journal of Agricultural Research, 7(5), 747-754.
Parker, C. (2012). Parasitic weeds: A World challenge. Weed Science, 60(2),269-276.
Bokosi, J., & Mumba, P. (2019). Screening of Alectra vogelii ecotypes on legume and non-legume crop species in Malawi. South African Journal of Plant and Soil, 36(2), 137-142.
Ranganathan, J., Waite, R., Searchinger, T.,& Hanson, C. (2018). Howto sustainably feed 10 billion people by 2050, in 21 charts.
Ricciardi, L. (2016). Characterization of low-Riches, C. R., Hamilton, K. A., & Parker, C. (1992). Parasitism of grain legumes by Alectra species (Scrophulariaceae). Annals of applied biology, 121(2), 361-370.
Ruyter-Spira, C. (2011). Strigolactones are transported through the xylemandplayakeyrole in shoot architectural response to phosphate deficiency in nonarbuscular mycorrhizal host Arabidopsis. Plant Physiology, 155 (2), 974-987.
Samejima, H., & Sugimoto, Y. (2018). Recent research progress in combatting root
Singh, B. B., & Emechebe, A. M. (1991). Breeding for resistance to Striga and Alectra in cowpea. In Proceedings of the 5th International symposium of parasitic weeds, Nairobi,
Singh, B. B., & Emechebe, A. M. (1997). Advances in research on cowpea Striga and Alectra. Advances in cowpea research. Copublication of International Institute of Tropical Agriculture (IITA) and Japan International Research Center for Agricultural Sciences (JIRCAS), IITA, Ibadan, Nigeria, 215-224.
strigolactone germplasm in pea (Pisum sativum L.) resistant to crenate broomrape (Orobanche crenata Forsk.). Molecular Plant-Microbe Interactions, 29(10), 743-749.
Ueno, K., Nomura, S., Xie, X., Yoneyama, K., & Yoneyama, K. (2010). The strigolactone Story. Annual review of phytopathology, 48, 93-117.
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